Importance of ISO and sterility shock
Posted: Fri Jul 11, 2008 1:48 am
If new crabs have come from poor condition (most pet stores), isolation is extremely important. Many crabbers have been lucky to get some healthier crabs and they have survived without being initially ISO'd. But overall, the chances of getting a new crab to survive past the first molt (averaged for all crabs for all crab owners) is significantly increased by using a graduated isolation period. Remember the effects of environmental stress could take many months to manifest. Any crab is still a "new" crab until they survive their first molt. Transferring from a good tank to a good tank (for instance, adoptions, or from the ISO to the main tank) are not a major problem. Stress isn't as much caused by a change in surroundings (different tank, different toys, different bowls, different shelters, etc). So transferring a crab from an ISO to a main tank isn't a big deal. So there isn’t much need to worry about adding stress from this second move in a short period (pet store to ISO, then ISO to main tank), and that definitely is not a reason to skip isolation.The big shock that we usually associate with new tanks or deep cleans is caused by sterility shock. The substrate and water have a certain chemical and biological balance to them. If you completely replace it all with new/clean sterile substrate, that causes environmental shock to them. So just make sure your ISO is seeded with active substrate from the main tank.That's why I recommend saving some of the old substrate to mix in with the new. And save a bit of the old water when changing a large pool as well. I save about 1/6th to 1/4 of the old substrate, and about 1/2 cup of water. It has been documented in threads before that there seems to be an increase in deaths shortly following a full deep clean, and this is suspected to be the cause.Reference:Coenobita.org (Sue) wrote two articles on the importance of ISO and minimizing PPS: http://coenobita.org/xoops/mod...les/article.php?id=4 http://coenobita.org/xoops/mod...es/article.php?id=14 However, coenobita.org seems to be down for a couple weeks, not sure what happened to it. Around the same time Sue was working on those articles, I was experimenting with starting the ISO at lower humidity after a bout of healthy crabs dying from PPS. I wrote up the mini-caresheet on what I termed "Environmental Stress" and described my method of ISOing to minimize it. http://home.comcast.net/~jedim...PurchaseSyndrome.doc (Also linked in a sticky in the Zoea sub-forum. My document includes the coenobita.org links as reference). The methods look similar at first glance, but the details of what humidity to start at, how much to change, temperature changes, substrate depth, and ISO setup differ between the method I wrote up and the one that Sue describes. The best for you will be whatever works for you. My caresheet is really just a one-page description of my method of ISO. So definitely read the coenobita articles if you can find them, because those are multi-page descriptions of the "why" ISO is important and "why" PPS happens."Environmental Stress" and "Sterility Shock" are as far as I know my own terms and definitions. Other resources may differ in opinions.